Infectious Disease Research

Virology
Respiratory and Enteric Virus Research and Development

Anti-Enteric Virus Evaluation

• Primary screen: We have a moderate throughput cell-based CPE reduction assay to identify inhibitors of a variety of enteric viruses. This is a microtiter assay which measures the ability of selected compounds to inhibit virus induced cell killing as well as toxicity of the test compounds to host cells. In parallel with the primary screen the toxicity of the compounds to the target cells is evaluated by use of tetrazolium dye XTT.

We have a number of viral strains for many of the enteric virus group for the primary screen:

Enteric viruses:

Virus Group	Cell-Line	Assay Length
Enteroviruses (J670/71)	MRC-5	6 days
Echoviruses (HA-201468)	MRC-5	6 days
Coxsackie virus A and B (A21, A24, A7, B1, B3 and B4)	MRC-5/Vero	6 days
Polio Virus (chat)	Vero	6 days



• Range of action: Compounds are evaluated for their ability to inhibit the replication of different strains within a group of viruses (different strains of Coxsackie and Enteroviruses).

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Respiratory and Enteric
Enteric Viruses
Respiratory and Enteric Virus Web Resources

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